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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
10.
Molecular charecterization of avian adeno virus
causing inclusion body hepatitis-hydropericardium
syndrome in broiler chickens of Anand, Gujarat,
India -
K B Thakor, C J Dave, K S Prajapati, D T Fefar
and B
M Jivani
Vet World. 2012; 5(3): 178-182
doi:
10.5455/vetworld.201 2.178-182
Abstract
Avian Adenovirus was isolated from naturally
infected broiler chickens. Two Liversample were
collected in glycerol saline from the birds came
from Aman and Jankipoutry farm for the postmortem
in the Dept. of pathology,Veterinary
college,Anand(Gujarat). Extraction of viral DNA
from infected liver tissues was done as per the
method of Meuleman et al., (2001) with minor
modifications. The amplified PCR analyzed by
agarose gel electrophoresis indicated DNA
fragments of approximately 890 bp as expected by
primer HexonA F & HexonB R. PCR assay revealed
presence of IBH-HPS virus in both samples.
Obtained PCR product of both sample were subjected
to DNA sequencing and obtained sequencing was
compared with other matched sequince. On
phylogenetic analysis using Clustal W program
showed 3 major group like upper, middle and lower
respectively. In the minor branch of upper group
the AMAN and JANKI isolates were found to group
with Fowl adenovirus 12 strain 380 and Fowl
adenovirus 11 strain C2B, so AMAN and JANKI
isolates indicating a new fowl adenovirus
genotype.
Keywords: Hydropericardium
syndrome, Avian Adenovirus, Polymerase Chain
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